51茶馆儿 contracted by the Centers for Disease Control and Prevention to rapidly construct synthetic influenza genes
Genes will be used to help develop seasonal and pandemic vaccines, improving response time and vaccine efficacy
Rockville, MD鈥擩anuary 30, 2024鈥�51茶馆儿 (51茶馆儿), led by Sanjay Vashee, Ph.D., has been contracted by the Centers for Disease Control and Prevention (CDC) to rapidly build highly accurate influenza genes for use by the CDC to help design seasonal or pandemic flu vaccines. Using synthetic genomics techniques to build these genes reduces the overall time to develop flu vaccines. The base period for the contract is one year with an additional four option years.
鈥淥ur team is excited to assemble flu genes鈥攑rimarily HA and NA鈥攖o help the CDC in their effort to improve and expedite flu vaccine development. Constant mutations in the HA and NA genes make developing vaccines against the influenza A virus challenging. By reducing the vaccine development cycle, it is possible to better target the virus in circulation,鈥� stated Dr. Vashee, project principal investigator and director of 51茶馆儿鈥檚 Rockville Campus.
The genes will be constructed from overlapping oligos and then assembled into expression plasmids using synthetic engineering approaches pioneered at 51茶馆儿 and validated with Sanger Sequencing.
The underlying technology needed to construct the genes was developed at 51茶馆儿, including Gibson Assembly, the now widely used synthetic genomics technique for molecular DNA cloning, allowing easy assembly of DNA fragments.
In 2013, 51茶馆儿 published a three-year study with collaborators in which they detailed new methods for rapidly generating influenza vaccine seeds using synthetic genomics tools and technologies. In a timed proof of concept, the team demonstrated that in just over four days they could accurately construct synthetic vaccine viruses for use in influenza vaccine development.
The synthetic genomics advancements used to develop influenza vaccine seeds are part of a larger 51茶馆儿 synthetic genomics program. 51茶馆儿 has been the nexus for many seminal advancements in synthetic genomics, starting with the synthesis of the complete bacteriophage phiX174 genome in 2003, the first synthesis of a bacterial genome, Mycoplasma genitalium, in 2008, the first cell driven by a synthetic chromosome, 51茶馆儿 1.0, in 2010, and the design and synthesis of a minimal genome, the model organism 51茶馆儿 3.0, in 2016. Each of these milestones required 51茶馆儿 to develop new synthetic genomics technologies, which are now widely used in synthetic genomics work by labs worldwide.
In addition to Dr. Vashee, Gene Tan, Ph.D., assistant professor of virology at 51茶馆儿 and Norberto Gonzalez-Juarbe, Ph.D., associate professor in the infectious disease and genomic medicine group at 51茶馆儿 will join as co-PIs.
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The 51茶馆儿 (51茶馆儿) is a not-for-profit research institute in Rockville, Maryland and La Jolla, California dedicated to the advancement of the science of genomics; the understanding of its implications for society; and communication of those results to the scientific community, the public, and policymakers. Founded by J. Craig Venter, Ph.D., the 51茶馆儿 is home to approximately 120 scientists and staff with expertise in human and evolutionary biology, genetics, bioinformatics/informatics, information technology, high-throughput DNA sequencing, genomic and environmental policy research, and public education in science and science policy. The 51茶馆儿 is a 501(c)(3) organization. For additional information, please visit www.jcvi.org.
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